DNA checkpoints in fission yeast
نویسندگان
چکیده
منابع مشابه
DNA checkpoints in fission yeast.
The DNA integrity checkpoints ensure completion of DNA replication and DNA repair before entry into mitosis (O’Connell et al., 2000). Unreplicated DNA or unrepaired DNA will cause catastrophic chromosome segregation. The accompanying schematic shows the checkpoint proteins that monitor the state of DNA replication and DNA repair. Checkpoint proteins most probably interact with the replication c...
متن کاملA novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints.
To further dissect the genetic differences between the checkpoint pathway following S-phase cdc arrest versus DNA damage, a genetic screen was performed for checkpoint mutants that were unable to arrest mitosis following cell-cycle arrest with a temperature-sensitive DNA polymerase delta mutant, cdc20-M10. One such checkpoint mutant, rad17-d14, was found to display the cut phenotype following S...
متن کاملOrganization of DNA replication origins in the fission yeast genome.
Eukaryotic DNA replication initiates at multiple points along the chromosomes known as replication origins (ORIs). We have developed a strategy to identify ORIs directly from replication intermediates in the fission yeast Schizosaccharomyces pombe. Mapping of a selection of the novel ORIs onto the genome reveals their preferential localization at intergenic regions upstream from genes. These re...
متن کاملModeling the control of DNA replication in fission yeast.
A central event in the eukaryotic cell cycle is the decision to commence DNA replication (S phase). Strict controls normally operate to prevent repeated rounds of DNA replication without intervening mitoses ("endoreplication") or initiation of mitosis before DNA is fully replicated ("mitotic catastrophe"). Some of the genetic interactions involved in these controls have recently been identified...
متن کاملCDK Phosphorylation of Drc1 Regulates DNA Replication in Fission Yeast
Cyclin-dependent kinases (CDKs) are absolutely required for DNA replication in eukaryotic cells. CDKs are thought to activate one or more replication factors, but the identities of these proteins are unknown. Here we describe fission yeast Drc1, a protein required for DNA replication that is phosphorylated by Cdc2. Drc1 depletion leads to catastrophic mitotic divisions with incompletely replica...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 2003
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.00790